绵羊UCP2基因的克隆及其在脂肪组织中的季节性差异表达

doi:10.11843/j.issn.0366-6964.2015.07.006

Abstract

Abstract:

This study focused on the cloning of ovine UCP2 gene and its seasonal expression differences in adipose tissues.The results would reveal the effects of seasonal regulation on the UCP2 expression.The full-length cDNA was cloned by RACE method.Real-time PCR and immunohistochemistry techniques were used to study the expressions of UCP2 mRNA and its coding protein in 6 adipose tissues of South African Mutton Merino and Shanxi Mutton Sheep Dam Line during two season periods.The results showed that the ovine UCP2 cDNA was 1 641 bp in length with a 930 bp open reading frame.It contained 8 exons which encoded 309 amino acids.The mRNA expressed in all 6 adipose tissues detected in this study.Higher expression in deep than in superficial depots (P<0.05) was found as suggested by the highest expression in perirenal and lowest in subcutaneous fat.UCP2 expressed in similar trend at mRNA and protein levels，and distributed on the membrane of adipocytes.Both mRNA and protein expressions were higher in winter-spring period than that in summer-autumn period (P<0.05).These results indicate that UCP2 differentially expression in adipose tissues is related to seasonal changes and functions in maintaining body temperature and regulating energy balance.

CLC Number:

S826.2

LIU Jian-hua，QIAO Li-ying，GUO Yun-yan，JIA Xia-li，LIU Wen-zhong. Cloning of Ovine UCP2 Gene and Its Seasonally Differential Expression in Adipose Tissues[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(7): 1114-1121.

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References

［1］RICQUIER D，BOUILLAUDAUD F.Mitochondrial uncoupling roreins：from mitochondria to the regulation of energy balance［J］.J Physiol，2000，529：3-10.
［2］FLEURY C，NEVEROV A，COLLINS S，et al.Uncoupling protein 2：a novel gene linked to obesity and hyperinemia［J］.Nat Genet，1997，15：269-272.
［3］RICQUIER D，BOUILLAUD F.The uncoupling protein homologues：UCP1，UCP2，UCP3，StUCP and AtUCP［J］.Biochem J，2000，345：161-179.
［4］CHA M H，KIM I C，KIM K S，et al.Association of UCP2 and UCP3 gene polymorphisms with serum high-density lipoprotein cholesterol among Korean women［J］.Metabolism，2007，56(6)：806-813.
［5］DALGAARD L T.Genetic variance in uncoupling protein 2 in relation to obesity，type 2 diabetes，and related metabolic traits：Focus on the functional -866G>A promoter variant (rs659366)［J］.J Obes，2011，34(2)：241-247.
［6］LI H，LI Y，ZHAO X，et al.Structure and nucleotide polymorphisms in pig uncoupling protein 2 and 3 gene［J］.Anim Biotechnol，2005，16(2)：209-220.
［7］SHERMAN E L，NKRUMAH J D，MURDOCH B M，et al.Polymorphisms and haplotypes in the bovine neuropeptide Y，growth hormone receptor，ghrelin，insulin-like growth factor 2，and uncoupling proteins 2 and 3 genes and their associations with measures of growth，performance，feed efficiency，and carcass merit in beef cattle［J］.J Anim Sci，2008，86(1)：1-16.
［8］WU B，FENG Y H，LIU C B，et al.Effects of repeated fasting/refeeding on lipid metabolism and uncoupling proteins expression in rats［J］.Zhongguo Ying Yong Sheng Li Xue Za Zhi，2013，29(4)：363-367.
［9］MOON J S，LEE S，PARK M A，et al.UCP2-induced fatty acid synthase promotes NLRP3 inflammasome activation during sepsis［J］. J Clin Invest，2015，125(2)：665-680.
［10］DE QUEIROZ K B 1，GUIMARAES J B，COIMBRA C C，et al.Endurance training increases leptin expression in the retroperitoneal adipose tissue of rats fed with a high-sugar diet［J］.Lipids，2014，49(1)：85-96.
［11］LI N，WANG J，GAO F，et al.The role of uncoupling protein 2 in the apoptosis induced by free fatty acid in rat cardiomyocytes［J］.J Cardiovasc Pharmacol，2010，55(2)：161-167.
［12］HENRY B A，ANDREWS Z B，RAO A，et al.Central Leptin Activates Mitochondrial Function and Increases Heat Production in Skeletal Muscle［J］.Endocrinology，2011，152(7)：2609-2618.
［13］LUO G F，YU T Y，WEN X H，et al.Alteration of mitochondrial oxidative capacity during porcine preadipocyte differentiation and in response to leptin［J］.Mol Cell Biochem，2008，307(1-2)：83-91.
［14］AGUER C，HARPER M E.Skeletal muscle mitochondrial energetics in obesity and type 2 diabetes mellitus：Endocrine aspects［J］.Best Pract Res Clin Endocrinol Meta，2012，26 (6)：805-819.
［15］TATA J R.The road to nuclear receptors of thyroid hormone［J］. Biochim Biophys Acta，2013，1830(7)：3860-3866.
［16］RAMSAY T G，ROSEBROUGH R W.Regulation of uncoupling proteins 2 and 3 in porcine adipose tissue［J］.Domest Anim Endocrinol，2005，28(4)：351-366.
［17］袁亚男，刘文忠，刘建华，等.绵羊UCP1基因碱基突变与其蛋白结构和功能［J］.中国农业科学，2012，45(14)：2973-2980.
YUAN Y N，LIU W Z，LIU J H，et al.The base mutation of ovine UCP1 gene and its relationship with protein structure and function［J］.Scientia Agricultura Sinica，2012，45(14)：2973-2980.(in Chinese)
［18］周沙沙，刘宝凤，魏琳琳，等.绵羊UCP4基因编码序列的克隆和mRNA表达研究［J］.中国农业科学，2013，46(10)：2110-2118 .
ZHOU S S，LIU B F，WEI L L，et al.Study on the cloning of coding sequence and ontogenetic mRNA expression of ovine UCP4 gene［J］.Scientia Agricultura Sinica，2013，46 (10)：2110-2118.(in Chinese)
［19］周沙沙.绵羊UCP4和UCP5基因的克隆、表达、多态性及其与性状的关联研究［D］.太谷：山西农业大学，2013.
ZHOU S S.Study on the cloning，expression and polymorphism of UCP4 and UCP5 gene and their associations with traits in sheep［D］.Taigu：Shanxi Agricultural University，2013.(in Chinese)
［20］KOLATH W H，KERLEY M S，GOLDEN J W，et al.The relationships among mitochondrial uncoupling protein 2 and 3 expression，mitochondrial deoxyribonucleic acid single nucleotide polymorphisms，and residual feed intake in Angus steers［J］.J Anim Sci，2006，84 (7)：1761-1766.
［21］CAO T，DONG Y，TANG R，et al.Mitochondrial uncoupling protein 2 protects splenocytes from oxidative stress-induced apoptosis during pathogen activation［J］.Cell Immunol，2013，286(1-2)：39-44.
［22］ESTEVES P，PECQUEUR C，RANSY C，et al.Mitochondrial retrograde signaling mediated by UCP2 inhibits cancer cell proliferation and tumorigenesis［J］.Cancer Res，2014，74(14)：3971-3982.
［23］ALAN L，SMOLKOVA K，KRONUSOVA E，et al.Absolute levels of transcripts for mitochondrial uncoupling proteins UCP2，UCP3，UCP4，and UCP5 show different patterns in rat and mice tissues［J］.J Bioenerg Biomembr，2009，41(1)：71-78.
［24］YANG L，LU K，WEN X Y，et al.Jueming Prescription reduces body weight by increasing the mRNA expressions of beta3-adrenergic receptor and uncoupling protein-2 in adipose tissue of diet-induced obese rats［J］. Chin J Integr Med，2012，18(10)：775-781.
［25］MAHADIK S R，LELE R D，SARANATH D，et al.Uncoupling protein-2 (UCP2) gene expression in subcutaneous and omental adipose tissue of Asian Indians：Relationship to adiponectin and parameters of metabolic syndrome［J］.Adipocyte，2012，1(2)：101-107.
［26］OBERKOFLER H，LIU Y M，ESTERBAUER H，et al.Uncoupling protein-2 gene：reduced mRNA expression in intraperitoneal adipose tissue of obese humans［J］.Diabetologia，1998，41(8)：940-946.
［27］HOPE P J，TURNBULL H，BREED W，et al.The effect of ovarian steroids and photoperiod on body fat stores and uncoupling protein 2 in the marsupial Sminthopsis crassicaudata［J］.Physiol Behav，2000，69(4-5)：463-470.
［28］SABIR S，CHAU S，HAMILTON J，et al.Seasonal cues & hibernation alter expression of uncoupling proteins in the golden hamster［J］.FASEB J，2006，20 (Meeting Abstract Supplement)：A828.
［29］ALLISTER E M，ROBSON-DOUCETTE C A，PRENTICE K J，et al.UCP2 regulates the glucagon response to fasting and starvation［J］.Diabetes，2013，62(5)：1623-1633.
［30］ARSENIJEVIC D，ONUMA H，PECQUEUR C，et al.Disruption of the uncoupling protein-2 gene in mice reveals a role in immunity and reactive oxygen species production［J］.Nat Genet，2000，26(4)：435-439.
［31］ABDELHAMID R E，KOVACS K J，NUNEZ M G，et al.After a cold conditioning swim，UCP2-deficient mice are more able to defend against the cold than wild type mice［J］.Physiol Behav，2014，135：168-173.
［32］NISHIMURA T，WATANUKI S.Relationship between mitochondrial haplogroup and seasonal changes of physiological responses to cold［J］. J Physiol Anthropol，2014，33：27.

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Cloning of Ovine UCP2 Gene and Its Seasonally Differential Expression in Adipose Tissues

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